Composite

Part:BBa_K4614107:Design

Designed by: Qianhui Sun   Group: iGEM23_CAU-China   (2023-10-06)


T7 Promoter-RBS-LuxR-B0015 Ter+Plux-RBS-sfGFP-B0015 Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 969


Design Notes

Importing this pathway into a low-copy-number vector may result in Plux promoter leakage due to insufficient LuxR production. Conversely, the strong T7 promoter could lead to issues such as inclusion body formation and high signal molecule concentration requirements for fluorescence.


Source

The T7, B0015, J23101, and the RBS sequences can all be found in the part library. The luxR gene can be obtained by designing primers and conducting PCR from Vibrio fischeri.The improved sfGFP gene can be obtained through base synthesis. Our team's luxR gene and sfGFP gene were generously provided as plasmids from ShanghaiTech_China 2023.

References